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2′,2′-Ligation Demonstrates the Thermal Dependence of DNA-Directed Positional Control
An oligodeoxynucleotide (ODN) containing a 2′-aminoalkylmercaptouridine was ligated chemically with another ODN containing a 2′-carboxyalkylmercaptouridine to produce a 2′,2′-amide linked conjugate. Templation by a DNA hairpin resulted in ligation yields of > 95%. At room temperature, similar yie...
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| Main Authors: | , , , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
2008
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2583448/ https://ncbi.nlm.nih.gov/pubmed/19122844 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.tet.2008.05 |
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