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Monitoring the reaction of carbachol with acetylcholinesterase by thioflavin T fluorescence and acetylthiocholine hydrolysis

Acetylcholinesterase (AChE) contains a narrow and deep active site gorge with two sites of ligand binding, an acylation site (or A-site) at the base of the gorge and a peripheral site (or P-site) near the gorge entrance. The P-site contributes to catalytic efficiency by transiently binding substrate...

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Autori principali: Rosenberry, Terrone L., Sonoda, Leilani K., Dekat, Sarah E., Cusack, Bernadette, Johnson, Joseph L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2583434/
https://ncbi.nlm.nih.gov/pubmed/18602908
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.cbi.2008.06.002
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