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Analysis of the reaction of carbachol with acetylcholinesterase with thioflavin T as a coupled fluorescence reporter

Acetylcholinesterase (AChE) contains a narrow and deep active site gorge with two sites of ligand binding, an acylation site (or A-site) at the base of the gorge and a peripheral site (or P-site) near the gorge entrance. The P-site contributes to catalytic efficiency by transiently binding substrate...

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Detalhes bibliográficos
Main Authors: Rosenberry, Terrone L., Sonoda, Leilani K., Dekat, Sarah E., Cusack, Bernadette, Johnson, Joseph L.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2655144/
https://ncbi.nlm.nih.gov/pubmed/19006330
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi8015197
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