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Streptococcus pneumoniae produces at least two distinct enzymes with neuraminidase activity: cloning and expression of a second neuraminidase gene in Escherichia coli.

A gene from Streptococcus pneumoniae was cloned in lambda EMBL301 and then expressed in Escherichia coli, which cleaved the fluorogenic neuraminidase substrate 2'-(4-methylumbelliferyl)-alpha-d-N-acetylneuraminic acid. The cloned gene therefore encodes an enzyme with neuraminidase activity. On...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:Infect Immun
Hauptverfasser: Camara, M, Mitchell, T J, Andrew, P W, Boulnois, G J
Format: Artigo
Sprache:Inglês
Veröffentlicht: American Society for Microbiology (ASM) 1991
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC258100/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1856002/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.59.8.2856-2858.1991
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