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Isolation and detection of urease genes in Ureaplasma urealyticum.

Urease from ureaplasmas was purified by immunoaffinity chromatography, and the N-terminal amino acid sequence was determined for two of the three subunits. These sequences were used to design primers for a polymerase chain reaction (PCR) that amplified most of the gene coding for one of the subunits...

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Detalhes bibliográficos
Main Authors: Willoughby, J J, Russell, W C, Thirkell, D, Burdon, M G
Formato: Artigo
Idioma:Inglês
Publicado em: 1991
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC258032/
https://ncbi.nlm.nih.gov/pubmed/2050410
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