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Isolation and detection of urease genes in Ureaplasma urealyticum.

Urease from ureaplasmas was purified by immunoaffinity chromatography, and the N-terminal amino acid sequence was determined for two of the three subunits. These sequences were used to design primers for a polymerase chain reaction (PCR) that amplified most of the gene coding for one of the subunits...

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Bibliografiske detaljer
Udgivet i:Infect Immun
Principais autores: Willoughby, J J, Russell, W C, Thirkell, D, Burdon, M G
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1991
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC258032/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2050410/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.59.7.2463-2469.1991
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