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Synthesis of tetanus toxin fragment C in insect cells by use of a baculovirus expression system.

The baculovirus expression vector p36C was used to express in cells of the insect Spodoptera frugiperda fragment C of tetanus toxin under the control of the strong polyhedrin promoter. Fragment C was expressed intracellularly at a high level and was soluble, allowing it to be purified by affinity ch...

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Bibliografiske detaljer
Udgivet i:Infect Immun
Principais autores: Charles, I G, Rodgers, B C, Makoff, A J, Chatfield, S N, Slater, D E, Fairweather, N F
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1991
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC257894/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2019434/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.59.5.1627-1632.1991
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