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Heterologous protein production in Escherichia coli using the propionate-inducible pPro system by conventional and auto-induction methods

We examined expression of two plant genes encoding coclaurine N-methyltransferase (CMT) and norcoclaurine synthase (NCS) in Escherichia coli from the Salmonella enterica prpBCDE promoter (P(prpB)) and compared it to that from the strongest IPTG-inducible promoter, P(T7). In contrast to our previous...

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Main Authors: Lee, Sung Kuk, Keasling, Jay D.
格式: Artigo
語言:Inglês
出版: 2008
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2577693/
https://ncbi.nlm.nih.gov/pubmed/18639640
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2008.06.008
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