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Inhibition of Aspergillus fumigatus elastase with monoclonal antibodies produced by using denatured elastase as an immunogen.

In preparing monoclonal antibodies to the elastase from Aspergillus fumigatus, we found that the enzyme was weakly immunogenic in BALB/c mice. Antiserum titers were only 1:1,000 to 1:5,000, and hybridomas secreted nonspecific immunoglobulin M (IgM). Denaturing the elastase in 0.5% sodium dodecyl sul...

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Udgivet i:Infect Immun
Principais autores: Frosco, M, Fahed, C, Chase, T, Macmillan, J D
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1992
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC257547/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1541546/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.60.3.735-741.1992
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