Inhibition of Aspergillus fumigatus elastase with monoclonal antibodies produced by using denatured elastase as an immunogen.

In preparing monoclonal antibodies to the elastase from Aspergillus fumigatus, we found that the enzyme was weakly immunogenic in BALB/c mice. Antiserum titers were only 1:1,000 to 1:5,000, and hybridomas secreted nonspecific immunoglobulin M (IgM). Denaturing the elastase in 0.5% sodium dodecyl sul...

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Detalhes bibliográficos
Publicado no:Infect Immun
Main Authors: Frosco, M, Fahed, C, Chase, T, Macmillan, J D
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1992
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC257547/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1541546/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/iai.60.3.735-741.1992
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