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Quenching-enhanced fluorescence titration protocol for accurate determination of free energy of membrane binding
Fluorescence spectroscopy is a convenient tool to examine peptide-membrane interactions at equilibrium (1) owing to the change in emission properties of many fluorophores (including tryptophan) during transfer from an aqueous environment into a lipid bilayer. In some cases (e.g. mechanosensitive cha...
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| Hauptverfasser: | , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2006
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2572995/ https://ncbi.nlm.nih.gov/pubmed/17184723 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2006.11.022 |
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