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Purification and Mass Spectrometric Analysis of the κ Opioid Receptor

A clonal human embryonic kidney (HEK) 293 cell line was established that stably expressed the rat κ-opioid receptor (rKOR) with a FLAG epitope at the amino terminus. The K(d) for [(3)H]diprenorphine was 1.1 ± 0.2 nM, and the B(max) was 2.6 ± 0.4 pmoles/mg. Dynorphin A (1–13), U69,593 and naloxone co...

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Detalhes bibliográficos
Main Authors: Wannemacher, Kenneth M., Terskiy, Alexandra, Bian, Shengjie, Yadav, Prem N., Li, Hong, Howells, Richard D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2570952/
https://ncbi.nlm.nih.gov/pubmed/18656460
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.brainres.2008.06.121
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