Phosphomimetic Mutations Increase Phospholamban Oligomerization and Alter the Structure of Its Regulatory Complex

To investigate the effect of phosphorylation on the interactions of phospholamban (PLB) with itself and its regulatory target, SERCA, we measured FRET from CFP-SERCA or CFP-PLB to YFP-PLB in live AAV-293 cells. Phosphorylation of PLB was mimicked by mutations S16E (PKA site) or S16E/T17E (PKA+CaMKII...

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Bibliographic Details
Main Authors: Hou, Zhanjia, Kelly, Eileen M., Robia, Seth L.
Format: Artigo
Language:Inglês
Published: American Society for Biochemistry and Molecular Biology 2008
Subjects:
Membrane Transport, Structure, Function, and Biogenesis
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2570860/
https://ncbi.nlm.nih.gov/pubmed/18708665
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M804782200
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