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Characterization of the spontaneous “aging” of the heme oxygenase from the pathological bacterium Neisseria meningitidis via cleavage of the C-terminus in contact with the substrate; Implications for functional studies and the crystal structure

Solution (1)H NMR spectroscopy and mass spectrometry are utilized to characterize the irreversible “aging” of native heme oxygenase from N. meningitidis, NmHO. 2D NMR characterization of the cyanide-inhibited substrate complex shows that the C-terminal interaction between Arg208His209 and the expose...

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Detalhes bibliográficos
Main Authors: Liu, Yangzhong, Ma, Li-Hua, Zhang, Xuhong, Yoshida, Tadashi, Satterlee, James D., La Mar, Gerd N.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2566967/
https://ncbi.nlm.nih.gov/pubmed/16548515
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0523097
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