Advantages of q-PCR as a method of screening for gene targeting in mammalian cells using conventional and whole BAC-based constructs

We evaluate here the use of real-time quantitative PCR (q-PCR) as a method for screening for homologous recombinants generated in mammalian cells from either conventional gene-targeting constructs or whole BAC-based constructs. Using gene-targeted events at different loci, we show that q-PCR is a hi...

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Détails bibliographiques
Auteurs principaux: Gómez-Rodríguez, Julio, Washington, Valance, Cheng, Jun, Dutra, Amalia, Pak, Evgenia, Liu, Pentao, McVicar, Daniel W., Schwartzberg, Pamela L.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2008
Sujets:
Methods Online
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2566865/
https://ncbi.nlm.nih.gov/pubmed/18710883
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn523
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