Quantitative Real-Time Reverse Transcription-PCR Analysis Reveals Stable and Prolonged Neurotoxin Cluster Gene Activity in a Clostridium botulinum Type E Strain at Refrigeration Temperature

The relative expression levels of six botulinum neurotoxin cluster genes in a group II Clostridium botulinum type E strain grown at 10 or 30°C were investigated using quantitative real-time reverse transcription-PCR. An enzyme-linked immunosorbent assay was used to confirm neurotoxin expression. Dis...

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Main Authors: Chen, Ying, Korkeala, Hannu, Lindén, Jere, Lindström, Miia
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2008
Assuntos:
Food Microbiology
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2565948/
https://ncbi.nlm.nih.gov/pubmed/18708513
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00469-08
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