A procedure for highly specific, sensitive, and unbiased whole-genome amplification

Highly specific amplification of complex DNA pools without bias or template-independent products (TIPs) remains a challenge. We have developed a method using phi29 DNA polymerase and trehalose and optimized control of amplification to create micrograms of specific amplicons without TIPs from down to...

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Библиографические подробности
Главные авторы: Pan, Xinghua, Urban, Alexander Eckehart, Palejev, Dean, Schulz, Vincent, Grubert, Fabian, Hu, Yiping, Snyder, Michael, Weissman, Sherman M.
Формат: Artigo
Язык:Inglês
Опубликовано: National Academy of Sciences 2008
Предметы:
Biological Sciences
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC2563063/
https://ncbi.nlm.nih.gov/pubmed/18832167
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0808028105
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