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A procedure for highly specific, sensitive, and unbiased whole-genome amplification
Highly specific amplification of complex DNA pools without bias or template-independent products (TIPs) remains a challenge. We have developed a method using phi29 DNA polymerase and trehalose and optimized control of amplification to create micrograms of specific amplicons without TIPs from down to...
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| Main Authors: | , , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2563063/ https://ncbi.nlm.nih.gov/pubmed/18832167 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0808028105 |
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