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pK(a) of residue 66 in Staphylococal nuclease. I. Insights from QM/MM simulations with conventional sampling

A combined quantum mechanical/molecular mechanical (QM/MM) potential function is used in a thermodynamic integration approach to calculate the pK(a) of residue 66 in two mutants (V66E, V66D) of Staphylococal nuclease relative to solution. Despite the similarity in chemical nature and experimentally...

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Detalhes bibliográficos
Main Authors: Ghosh, Nilanjan, Cui, Qiang
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2562727/
https://ncbi.nlm.nih.gov/pubmed/18540669
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/jp800168z
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