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Subdividing repressor function: DNA binding affinity, selectivity, and allostery can be altered by amino acid substitution of nonconserved residues in a LacI/GalR homologue

Many mutations that impact protein function occur at residues that do not directly contact ligand. To understand the functional contributions from the sequence that links the DNA-binding and regulatory domains of the LacI/GalR homologues, we have created a chimeric protein (LLhP), which comprises th...

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Détails bibliographiques
Auteurs principaux: Zhan, Hongli, Taraban, Marc, Trewhella, Jill, Swint-Kruse, Liskin
Format: Artigo
Langue:Inglês
Publié: 2008
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2561905/
https://ncbi.nlm.nih.gov/pubmed/18616293
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi800443k
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