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Engineered Recombinant Single-Chain Fragment Variable Antibody for Immunosensors

A recombinant single-chain fragment variable (scFv) antibody (designated A10B) was engineered to contain two histidines within the linker peptide used to join the scFv heavy and light chains. A piezoimmunosensor using the scFv was successfully developed. A10B scFv bound to the gold piezoimmunosensor...

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Bibliographic Details
Main Authors: Shen, Zhihong, Mernaugh, Raymond L., Yan, Heping, Yu, Lei, Zhang, Ying, Zeng, Xiangqun
Format: Artigo
Language:Inglês
Published: 2005
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC2560180/
https://ncbi.nlm.nih.gov/pubmed/16255580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac0507690
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