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Characterization of the Native and Denatured Herceptin by ELISA and QCM using a High-Affinity Single Chain Fragment Variable (scFv) Recombinant Antibody

Herceptin/Trastuzumab is a humanized IgG1κ light chain antibody used to treat some forms of breast cancer. A phage-displayed recombinant antibody library was used to obtain an scFv (designated 2B4) to a linear synthetic peptide representing Herceptin’s heavy chain CDR3. ELISAs and piezoimmunosensor/...

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Main Authors: Shang, Yuqin, Mernaugh, Ray, Zeng, Xiangqun
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3488427/
https://ncbi.nlm.nih.gov/pubmed/22934911
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac301235a
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