Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin.
Cp-1, a small virulent bacteriophage infecting Streptococcus pneumoniae, encodes its own lytic enzyme (CPL). A fragment of Cp-1 DNA containing the gene cpl coding for CPL was cloned and expressed in high amounts in Escherichia coli. CPL was purified to electrophoretic homogeneity by using affinity c...
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| Опубликовано в:: | J Virol |
|---|---|
| Главные авторы: | , , , , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
American Society for Microbiology (ASM)
1987
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC255702/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3298686/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.61.8.2573-2580.1987 |
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