Cloning, purification, and biochemical characterization of the pneumococcal bacteriophage Cp-1 lysin.

Cp-1, a small virulent bacteriophage infecting Streptococcus pneumoniae, encodes its own lytic enzyme (CPL). A fragment of Cp-1 DNA containing the gene cpl coding for CPL was cloned and expressed in high amounts in Escherichia coli. CPL was purified to electrophoretic homogeneity by using affinity c...

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Veröffentlicht in:	J Virol
Hauptverfasser:	García, J L, García, E, Arrarás, A, García, P, Ronda, C, López, R
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1987
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC255702/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3298686/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.61.8.2573-2580.1987
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