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A serum substitute for fed-batch culturing of hybridoma cells

We developed a substitute for serum to produce fed-batch cultures of hybridoma cells in serum-free medium and confirmed that the cells could be successfully cultivated this way. Our substitute consisted of 12 components. The specific production rates of lactate and ammonia, which are harmful byprodu...

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Detalhes bibliográficos
Main Authors: Shibuya, Keisuke, Haga, Ryoichi, Namba, Masaru
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2553663/
https://ncbi.nlm.nih.gov/pubmed/19003165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-008-9155-y
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