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A serum substitute for fed-batch culturing of hybridoma cells
We developed a substitute for serum to produce fed-batch cultures of hybridoma cells in serum-free medium and confirmed that the cells could be successfully cultivated this way. Our substitute consisted of 12 components. The specific production rates of lactate and ammonia, which are harmful byprodu...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Springer Netherlands
2008
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2553663/ https://ncbi.nlm.nih.gov/pubmed/19003165 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-008-9155-y |
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