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NADPH-cytochrome P450 oxidoreductase from the mosquito Anopheles minimus: kinetic studies and the influence of Leu86 and Leu219 on cofactor binding and protein stability(§)

NADPH-cytochrome c oxidoreductase from the mosquito Anopheles minimus lacking the first 55 amino acid residues was expressed in E. coli. The purified enzyme loses FMN, leading to an unstable protein and subsequent aggregation. To understand the basis for the instability, we constructed single and tr...

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Bibliographische Detailangaben
Hauptverfasser: Sarapusit, Songklod, Xia, Chuanwu, Misra, Ila, Rongnoparut, Pornpimol, Kim, Jung-Ja P.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2008
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2548334/
https://ncbi.nlm.nih.gov/pubmed/18539133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2008.05.012
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