NADPH-cytochrome P450 oxidoreductase from the mosquito Anopheles minimus: kinetic studies and the influence of Leu86 and Leu219 on cofactor binding and protein stability(§)

NADPH-cytochrome c oxidoreductase from the mosquito Anopheles minimus lacking the first 55 amino acid residues was expressed in E. coli. The purified enzyme loses FMN, leading to an unstable protein and subsequent aggregation. To understand the basis for the instability, we constructed single and tr...

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Main Authors: Sarapusit, Songklod, Xia, Chuanwu, Misra, Ila, Rongnoparut, Pornpimol, Kim, Jung-Ja P.
Format: Artigo
Jezik:Inglês
Izdano: 2008
Teme:
Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2548334/
https://ncbi.nlm.nih.gov/pubmed/18539133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.abb.2008.05.012
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