Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis

Several Lactococcus lactis strains produce the lantibiotic nisin. The dedicated enzymes NisB and NisC and the transporter NisT modify and secrete the ribosomally synthesized nisin precursor peptide. NisB can function in the absence of the cyclase NisC, yielding the dehydrated prenisin that lacks the...

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Main Authors: van den Berg van Saparoea, H. Bart, Bakkes, Patrick J., Moll, Gert N., Driessen, Arnold J. M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
Assuntos:
Enzymology and Protein Engineering
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2546625/
https://ncbi.nlm.nih.gov/pubmed/18621866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00342-08
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spelling pubmed-25466252009-03-01 Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis van den Berg van Saparoea, H. Bart Bakkes, Patrick J. Moll, Gert N. Driessen, Arnold J. M. Appl Environ Microbiol Enzymology and Protein Engineering Several Lactococcus lactis strains produce the lantibiotic nisin. The dedicated enzymes NisB and NisC and the transporter NisT modify and secrete the ribosomally synthesized nisin precursor peptide. NisB can function in the absence of the cyclase NisC, yielding the dehydrated prenisin that lacks the thioether rings. A kinetic analysis of nisin production by L. lactis NZ9700 demonstrated that the prenisin was released from the cell into the medium before the processing of the leader sequence occurred. Upon the deletion of nisC, the production of prenisin was reduced by 70%, while in the absence of nisB, the production of prenisin was nearly completely abolished. In cells lacking nisT, no secretion was observed, while the expression of nisABC in these cells resulted in considerable growth rate inhibition caused by the intracellular accumulation of active nisin. Overall, these data indicate that the efficiency of prenisin transport by NisT is markedly enhanced by NisB, suggesting a channeling mechanism of prenisin transfer between the nisin modification enzymes and the transporter. American Society for Microbiology (ASM) 2008-09 2008-07-11 /pmc/articles/PMC2546625/ /pubmed/18621866 http://dx.doi.org/10.1128/AEM.00342-08 Text en Copyright © 2008, American Society for Microbiology
institution US NLM
collection PubMed Central
language Inglês
format Artigo
topic Enzymology and Protein Engineering
spellingShingle Enzymology and Protein Engineering
van den Berg van Saparoea, H. Bart
Bakkes, Patrick J.
Moll, Gert N.
Driessen, Arnold J. M.
Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis
description Several Lactococcus lactis strains produce the lantibiotic nisin. The dedicated enzymes NisB and NisC and the transporter NisT modify and secrete the ribosomally synthesized nisin precursor peptide. NisB can function in the absence of the cyclase NisC, yielding the dehydrated prenisin that lacks the thioether rings. A kinetic analysis of nisin production by L. lactis NZ9700 demonstrated that the prenisin was released from the cell into the medium before the processing of the leader sequence occurred. Upon the deletion of nisC, the production of prenisin was reduced by 70%, while in the absence of nisB, the production of prenisin was nearly completely abolished. In cells lacking nisT, no secretion was observed, while the expression of nisABC in these cells resulted in considerable growth rate inhibition caused by the intracellular accumulation of active nisin. Overall, these data indicate that the efficiency of prenisin transport by NisT is markedly enhanced by NisB, suggesting a channeling mechanism of prenisin transfer between the nisin modification enzymes and the transporter.
author van den Berg van Saparoea, H. Bart
Bakkes, Patrick J.
Moll, Gert N.
Driessen, Arnold J. M.
author_facet van den Berg van Saparoea, H. Bart
Bakkes, Patrick J.
Moll, Gert N.
Driessen, Arnold J. M.
author_sort van den Berg van Saparoea, H. Bart
title Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis
title_short Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis
title_full Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis
title_fullStr Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis
title_full_unstemmed Distinct Contributions of the Nisin Biosynthesis Enzymes NisB and NisC and Transporter NisT to Prenisin Production by Lactococcus lactis
title_sort distinct contributions of the nisin biosynthesis enzymes nisb and nisc and transporter nist to prenisin production by lactococcus lactis
publisher American Society for Microbiology (ASM)
publishDate 2008
url https://ncbi.nlm.nih.gov/pmc/articles/PMC2546625/
https://ncbi.nlm.nih.gov/pubmed/18621866
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00342-08
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