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Cre/lox System and PCR-Based Genome Engineering in Bacillus subtilis
We have developed a fast and accurate method to engineer the Bacillus subtilis genome that involves fusing by PCR two flanking homology regions with an antibiotic resistance gene cassette bordered by two mutant lox sites (lox71 and lox66). The resulting PCR products were used directly to transform B...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
American Society for Microbiology (ASM)
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2546623/ https://ncbi.nlm.nih.gov/pubmed/18641148 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01156-08 |
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