Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila

A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sput...

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Pubblicato in:	J Clin Microbiol
Autori principali:	Herpers, Bjorn L., de Jongh, Bartelt M., van der Zwaluw, Kim, van Hannen, Erik J.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 2003
Soggetti:	Bacteriology
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC254366/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/14532229/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/JCM.41.10.4815-4816.2003
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