Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila

A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sput...

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Detalhes bibliográficos
Main Authors: Herpers, Bjorn L., de Jongh, Bartelt M., van der Zwaluw, Kim, van Hannen, Erik J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC254366/
https://ncbi.nlm.nih.gov/pubmed/14532229
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.41.10.4815-4816.2003
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