Rapid Detection and Estimation by Pyrosequencing of 23S rRNA Genes with a Single Nucleotide Polymorphism Conferring Linezolid Resistance in Enterococci

Pyrosequencing was used to detect rapidly and estimate the number of 23S rRNA genes with a G2576T mutation in 43 linezolid-resistant and -susceptible clinical isolates of enterococci. The method showed 100% concordance with PCR-restriction fragment length polymorphism for detecting isolates homozygo...

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Detalhes bibliográficos
Main Authors: Sinclair, Alistair, Arnold, Catherine, Woodford, Neil
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2003
Assuntos:
Mechanisms of Resistance
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC253796/
https://ncbi.nlm.nih.gov/pubmed/14576130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.47.11.3620-3622.2003
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