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Effects of Modified Digestion Schemes on the Identification of Proteins from Complex Mixtures
In shotgun proteomics, a complex protein mixture is digested to peptides, separated and identified by microcapillary liquid chromatography followed by tandem mass spectrometry (LC-MS-MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
2006
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2535816/ https://ncbi.nlm.nih.gov/pubmed/16512685 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr050315j |
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