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Effects of Modified Digestion Schemes on the Identification of Proteins from Complex Mixtures

In shotgun proteomics, a complex protein mixture is digested to peptides, separated and identified by microcapillary liquid chromatography followed by tandem mass spectrometry (LC-MS-MS). In this technology, complete protein digestion is often assumed. We show that, to the contrary, modifications to...

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Detalhes bibliográficos
Main Authors: Klammer, Aaron A., MacCoss, Michael J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2006
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2535816/
https://ncbi.nlm.nih.gov/pubmed/16512685
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/pr050315j
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