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Efficient Ends-Out Gene Targeting In Drosophila

In this report, we describe several approaches to improve the scalability and throughput of major genetic crosses in ends-out gene targeting. We generated new sets of targeting vectors and fly stocks and introduced a novel negative selection marker that drastically reduced the frequency of false-pos...

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Detalhes bibliográficos
Main Authors: Huang, Juan, Zhou, Wenke, Watson, Annie M., Jan, Yuh-Nung, Hong, Yang
Formato: Artigo
Idioma:Inglês
Publicado em: Genetics Society of America 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2535722/
https://ncbi.nlm.nih.gov/pubmed/18757917
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1534/genetics.108.090563
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