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Optimizing TiO(2)-based phosphopeptide enrichment for automated multidimensional liquid chromatography coupled to tandem mass spectrometry

An automated online multidimensional liquid chromatography system coupled to ESI-based tandem mass spectrometry was used to assess the effectiveness of TiO(2) in the enrichment of phosphopeptides from tryptic digests of protein mixtures. By monitoring the enrichment of phosphopeptides, an optimized...

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Main Authors: Cantin, Greg T., Shock, Teresa R., Park, Sung Kyu, Madhani, Hiten D., Yates, John R.
Format: Artigo
Jezik:Inglês
Izdano: 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2535607/
https://ncbi.nlm.nih.gov/pubmed/17523591
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac0618730
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