Expression of herpes simplex virus type 1 DNA polymerase in Saccharomyces cerevisiae and detection of virus-specific enzyme activity in cell-free lysates.

The herpes simplex virus type 1 (HSV-1) (strain 17) DNA polymerase gene has been cloned into an Escherichia coli-yeast shuttle vector fused to the galactokinase gene (GAL-1) promoter. Genes controlled by the GAL-1 promoter are induced by galactose, uninduced by raffinose, and repressed by glucose. C...

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Pubblicato in:	J Virol
Autori principali:	Haffey, M L, Stevens, J T, Terry, B J, Dorsky, D I, Crumpacker, C S, Wietstock, S M, Ruyechan, W T, Field, A K
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1988
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC253559/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2846866/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.62.12.4493-4498.1988
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