Expression of herpes simplex virus type 1 DNA polymerase in Saccharomyces cerevisiae and detection of virus-specific enzyme activity in cell-free lysates.

The herpes simplex virus type 1 (HSV-1) (strain 17) DNA polymerase gene has been cloned into an Escherichia coli-yeast shuttle vector fused to the galactokinase gene (GAL-1) promoter. Genes controlled by the GAL-1 promoter are induced by galactose, uninduced by raffinose, and repressed by glucose. C...

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Détails bibliographiques
Publié dans:J Virol
Auteurs principaux: Haffey, M L, Stevens, J T, Terry, B J, Dorsky, D I, Crumpacker, C S, Wietstock, S M, Ruyechan, W T, Field, A K
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 1988
Sujets:
Research Article
Accès en ligne:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC253559/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2846866/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.62.12.4493-4498.1988
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