A new real-time PCR method to overcome significant quantitative inaccuracy due to slight amplification inhibition

BACKGROUND: Real-time PCR analysis is a sensitive DNA quantification technique that has recently gained considerable attention in biotechnology, microbiology and molecular diagnostics. Although, the cycle-threshold (Ct) method is the present "gold standard", it is far from being a standard...

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Main Authors: Guescini, Michele, Sisti, Davide, Rocchi, Marco BL, Stocchi, Laura, Stocchi, Vilberto
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2008
Fag:
Methodology Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2533027/
https://ncbi.nlm.nih.gov/pubmed/18667053
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-9-326
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