The stacking tryptophan of galactose oxidase: a second coordination sphere residue that has profound effects on tyrosyl radical behavior and enzyme catalysis

The function of the stacking tryptophan, W290, a second coordination sphere residue in galactose oxidase has been investigated via steady-state kinetics measurements, absorption, CD and EPR spectroscopy, and x -ray crystallography of the W290F, W290G, and W290H variants. Enzymatic turnover is signif...

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Main Authors: Rogers, Melanie S., Tyler, Ejan M., Akyumani, Nana, Kurtis, Christian R., Spooner, R. Kate, Deacon, Sarah E., Tamber, Sunita, Firbank, Susan J., Mahmoud, Khaled, Knowles, Peter F., Phillips, Simon E. V., McPherson, Michael J., Dooley, David M.
Formato: Artigo
Idioma:Inglês
Publicado: 2007
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2532978/
https://ncbi.nlm.nih.gov/pubmed/17385891
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi062139d
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