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Development of a fluorescent substrate to measure hyaluronidase activity
A novel fluorescent substrate (termed FRET-HA) to quantitatively assess hyaluronidase activity was developed. Hyaluronan (HA), the major substrate for hyaluronidase, was dual labeled with fluorescein amine and rhodamine B amine. The fluorescein amine fluorescence signal was significantly quenched wh...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2532756/ https://ncbi.nlm.nih.gov/pubmed/18492482 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.04.040 |
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