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Purification and partial characterization of poliovirus protease 2A by means of a functional assay.

The purification of poliovirus protease 2A from infected cells by a functional assay is described. A small synthetic peptide was cleaved specifically by an esterase present in poliovirus-infected cells. Since the enzyme proved extremely unstable in crude extracts a rapid and efficient purification p...

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Detalhes bibliográficos
Publicado no:J Virol
Main Authors: König, H, Rosenwirth, B
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC253133/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2831385/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jvi.62.4.1243-1250.1988
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