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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2)

A non-Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.0 Å resolu...

תיאור מלא

שמור ב:
מידע ביבליוגרפי
Main Authors: Wang, Shu, He, Yong-Xing, Bao, Rui, Teng, Yan-Bin, Ye, Bo-Ping, Zhou, Cong-Zhao
פורמט: Artigo
שפה:Inglês
יצא לאור: International Union of Crystallography 2008
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC2531269/
https://ncbi.nlm.nih.gov/pubmed/18765920
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910802575X
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