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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of hypothetical protein SCO4226 from Streptomyces coelicolor A3(2)
A non-Pfam hypothetical protein SCO4226 of molecular weight 9 kDa from Streptomyces coelicolor A3(2) was overexpressed in Escherichia coli and the purified recombinant protein was crystallized using the sitting-drop vapour-diffusion method. An X-ray diffraction data set was collected to 2.0 Å resolu...
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Main Authors: | , , , , , |
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Format: | Artigo |
Sprog: | Inglês |
Udgivet: |
International Union of Crystallography
2008
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Fag: | |
Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2531269/ https://ncbi.nlm.nih.gov/pubmed/18765920 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S174430910802575X |
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