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Fluorescence Recovery Kinetic Analysis of γ-Tubulin Binding to the Mitotic Spindle

Fluorescence recovery after photobleaching has been widely used to study dynamic processes in the cell, but less frequently to analyze binding interactions and extract binding constants. Here we use it to analyze γ-tubulin binding to the mitotic spindle and centrosomes to determine the role of γ-tub...

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Autori principali: Hallen, Mark A., Ho, Jianghai, Yankel, Christine D., Endow, Sharyn A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Biophysical Society 2008
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2527240/
https://ncbi.nlm.nih.gov/pubmed/18567627
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.134593
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