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Fluorescence Recovery Kinetic Analysis of γ-Tubulin Binding to the Mitotic Spindle
Fluorescence recovery after photobleaching has been widely used to study dynamic processes in the cell, but less frequently to analyze binding interactions and extract binding constants. Here we use it to analyze γ-tubulin binding to the mitotic spindle and centrosomes to determine the role of γ-tub...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Biophysical Society
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2527240/ https://ncbi.nlm.nih.gov/pubmed/18567627 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.108.134593 |
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