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Direct method for quantitation of extreme polymerase error frequencies at selected single base sites in viral RNA.

Methods are described which allow direct quantitation and sequence analysis of base substitution levels at predetermined single nucleotide positions in cloned pools of an RNA virus genome or in its RNA transcripts in vitro. Base substitution frequencies for vesicular stomatitis virus (VSV) at one hi...

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Detalhes bibliográficos
Main Authors: Steinhauer, D A, Holland, J J
Formato: Artigo
Idioma:Inglês
Publicado em: 1986
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC252718/
https://ncbi.nlm.nih.gov/pubmed/3001347
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