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Kinetic and Structural Insight into the Mechanism of BphD, a C-C Bond Hydrolase from the Biphenyl Degradation Pathway
Kinetic and structural analyses of 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA) hydrolase from Burkholderia xenovorans LB400 (BphD(LB400)) provide insight into the catalytic mechanism of this unusual serine hydrolase. Single turnover stopped-flow analysis at 25 °C showed that the enzyme rap...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2006
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2519953/ https://ncbi.nlm.nih.gov/pubmed/16964968 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi0611098 |
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