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Development and Validation of a Negative-Strand-Specific Reverse Transcription-PCR Assay for Detection of a Chicken Strain of Hepatitis E Virus: Identification of Nonliver Replication Sites

As a positive-strand RNA virus, hepatitis E virus (HEV) produces an intermediate negative-strand RNA when it replicates. Thus, the detection of negative-strand viral RNA is indicative of HEV replication. The objective of this study was to develop a negative-strand-specific reverse transcription-PCR...

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書誌詳細
主要な著者: Billam, P., Pierson, F. W., Li, W., LeRoith, T., Duncan, R. B., Meng, X. J.
フォーマット: Artigo
言語:Inglês
出版事項: American Society for Microbiology (ASM) 2008
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2519487/
https://ncbi.nlm.nih.gov/pubmed/18562592
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.00536-08
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