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Detection of Negative-Sense RNA in Packaged Hepatitis E Virions by Use of an Improved Strand-Specific Reverse Transcription-PCR Method

Current hepatitis E virus (HEV) negative-sense RNA detection assays have the drawback of false positivity. cDNA synthesis using tag-based primer and Superscript RT-III followed by exonuclease I treatment increased the specificity. Assays could detect as few as 10 copies of negative-sense RNA and cou...

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מידע ביבליוגרפי
Main Authors: Chatterjee, Subhashis N., Devhare, Pradip B., Lole, Kavita S.
פורמט: Artigo
שפה:Inglês
יצא לאור: American Society for Microbiology 2012
נושאים:
גישה מקוונת:https://ncbi.nlm.nih.gov/pmc/articles/PMC3318542/
https://ncbi.nlm.nih.gov/pubmed/22205803
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.06717-11
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