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Evaluation of PCR Primer Selectivity and Phylogenetic Specificity by Using Amplification of 16S rRNA Genes from Betaproteobacterial Ammonia-Oxidizing Bacteria in Environmental Samples

The effect of primer specificity for studying the diversity of ammonia-oxidizing betaproteobacteria (βAOB) was evaluated. βAOB represent a group of phylogenetically related organisms for which the 16S rRNA gene approach is especially suitable. We used experimental comparisons of primer performance w...

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Detalles Bibliográficos
Main Authors: Junier, Pilar, Kim, Ok-Sun, Hadas, Ora, Imhoff, Johannes F., Witzel, Karl-Paul
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 2008
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2519257/
https://ncbi.nlm.nih.gov/pubmed/18567688
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.00288-08
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